Quantitative tissue factor gene expression analysis in whole blood: development and evaluation of a real-time PCR platform.

نویسندگان

  • Jens Mueller
  • Jutta Maria Rox
  • Katharina Madlener
  • Bernd Poetzsch
چکیده

Whole Blood: Development and Evaluation of a RealTime PCR Platform, Jens Mueller, Jutta Maria Rox, Katharina Madlener, and Bernd Poetzsch ( Institute for Experimental Haematology and Transfusion Medicine, University of Bonn, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany; 2 Department of Haemostaseology and Transfusion Medicine, Kerckhoff-Klinik, Bad Nauheim, Germany; * author for correspondence: fax 49-228-2879090, e-mail [email protected])

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

P-182: The Role of Vascular Endothelial Growth Factor Gene Expression in Patients with the History of Endometriosis

Background: Endometriosis is the presence of endometrium- like tissue in sites outside the uterine cavity, primarily on the pelvic peritoneum and ovaries. Ectopic endometrium for replacement and growth require to blood supply. Vascular endothelial growth factor (VEGF) is one of the most important intermediate of locality angiogenesis that product by monocytes and macrophages. This study evaluat...

متن کامل

Evaluation of GNMT Gene Expression in Prostate Cancer Tissues using Real-Time PCR

Introduction: Prostate cancer is the second most common cancer and the leading cause of cancer-related deaths worldwide. In the present study, the expression level of glycine N-methyl transferase gene (GNMT) was investigated in prostate cancer tissue. The GNMT enzyme is encoded by the GNMT gene. Increased GNMT gene expression increases the conversion of glycine to sarcosine and results in the e...

متن کامل

Investigating the CTGF mRNA Expression Level in Patients with Colorectal Cancer

Background: The Connective Tissue Growth Factor (CTGF) gene encoding an extracellular matrix (ECM)-associated protein and as a member of the CCN family of proteins plays a major role in fibrosis, inflammation and connective tissue remodeling in a variety of diseases including cancer. The CCN proteins are multifunctional and are involved in cell proliferation, adhesion and cell development durin...

متن کامل

Evaluation of MALAT-1 and HOTAIR LncRNAs Expression Levels in Incidence of Hemophilia

Background and purpose: LncRNAs are a group of non-coding RNAs with more than 200 nucleotides involved in transcriptional and post-transcriptional regulation of gene expression, that have a potential role in diagnosis, prevention and treatment of some disorders such as cancers. There is paucity of information about their involvement in hemophilia disease. This study aimed at discovering the rel...

متن کامل

Selection of suitable reference genes for real-time PCR studies of early developmental stages of sturgeons

In quantitative real-time PCR, the mRNA level can be quantified in relative terms based on the expression ratio of mRNAs of the target gene and an internal reference gene. Since, an internal standard should be expressed at a constant level among different tissues of an organism at all stages of development, and should be unaffected by the experimental treatment, the stability of different refer...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Clinical chemistry

دوره 50 1  شماره 

صفحات  -

تاریخ انتشار 2004